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Image Search Results
Journal: Frontiers in Microbiology
Article Title: Yersiniabactin produced by Escherichia coli promotes intestinal inflammation through lipid peroxidation and ferroptosis
doi: 10.3389/fmicb.2025.1542801
Figure Lengend Snippet: Ybt induces ferroptosis in intestinal epithelial cells. (A) KEGG analysis was employed to investigate the association between Ybt and ferroptosis. (B) Volcano plots were generated to compare differential fold changes and p -values for individual genes after different treatments. (C) Clustering heatmaps were created to visualize the DEGs associated with ferroptosis after different treatments. (D,E) Western blot was used to detect the expression levels of iron death marker proteins after different treatments, including ACSL4, SLC7A11, GPX4, and SLC7A1, at the same time, the protein expression was quantitatively analyzed. (F–J) Intracellular lipid peroxide content was quantified at various time points following diverse treatments, including LPO, CAT, GSH-Px, MDA and SOD, n = 3. (K) The impact of the ferroptosis inhibitor Fer-1 on cell viability was evaluated following various treatments, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
Article Snippet: Cellular lipid peroxidation levels were assessed using specific lipid peroxidation detection kits, namely
Techniques: Generated, Western Blot, Expressing, Marker